Despite difficulties during practice, including a huge crash that left him with both shoulder and knee problems, Casey Stoner (Honda) came through with the fastest lap during qualifying for the Assen race tomorrow. Second and third quickest were Dani Pedrosa (Honda) and points leader Jorge Lorenzo (Yamaha).
| Pos. | Num. | Rider | Nation | Team | Bike | Km/h | Time | Gap 1st/Prev. |
| 1 | 1 | Casey STONER | AUS | Repsol Honda Team | Honda | 303.4 | 1’33.713 | |
| 2 | 26 | Dani PEDROSA | SPA | Repsol Honda Team | Honda | 303.4 | 1’33.828 | 0.115 / 0.115 |
| 3 | 99 | Jorge LORENZO | SPA | Yamaha Factory Racing | Yamaha | 305.7 | 1’34.001 | 0.288 / 0.173 |
| 4 | 6 | Stefan BRADL | GER | LCR Honda MotoGP | Honda | 304.0 | 1’34.035 | 0.322 / 0.034 |
| 5 | 35 | Cal CRUTCHLOW | GBR | Monster Yamaha Tech 3 | Yamaha | 299.0 | 1’34.486 | 0.773 / 0.451 |
| 6 | 11 | Ben SPIES | USA | Yamaha Factory Racing | Yamaha | 297.2 | 1’34.644 | 0.931 / 0.158 |
| 7 | 4 | Andrea DOVIZIOSO | ITA | Monster Yamaha Tech 3 | Yamaha | 299.6 | 1’34.698 | 0.985 / 0.054 |
| 8 | 19 | Alvaro BAUTISTA | SPA | San Carlo Honda Gresini | Honda | 299.4 | 1’34.722 | 1.009 / 0.024 |
| 9 | 69 | Nicky HAYDEN | USA | Ducati Team | Ducati | 300.0 | 1’34.751 | 1.038 / 0.029 |
| 10 | 46 | Valentino ROSSI | ITA | Ducati Team | Ducati | 302.1 | 1’35.057 | 1.344 / 0.306 |
| 11 | 8 | Hector BARBERA | SPA | Pramac Racing Team | Ducati | 305.0 | 1’35.289 | 1.576 / 0.232 |
| 12 | 14 | Randy DE PUNIET | FRA | Power Electronics Aspar | ART | 286.6 | 1’35.830 | 2.117 / 0.541 |
| 13 | 41 | Aleix ESPARGARO | SPA | Power Electronics Aspar | ART | 286.3 | 1’36.007 | 2.294 / 0.177 |
| 14 | 51 | Michele PIRRO | ITA | San Carlo Honda Gresini | FTR | 283.2 | 1’36.647 | 2.934 / 0.640 |
| 15 | 54 | Mattia PASINI | ITA | Speed Master | ART | 284.7 | 1’36.943 | 3.230 / 0.296 |
| 16 | 9 | Danilo PETRUCCI | ITA | Came IodaRacing Project | Ioda | 270.0 | 1’36.967 | 3.254 / 0.024 |
| 17 | 17 | Karel ABRAHAM | CZE | Cardion AB Motoracing | Ducati | 302.8 | 1’37.110 | 3.397 / 0.143 |
| 18 | 68 | Yonny HERNANDEZ | COL | Avintia Blusens | BQR | 279.8 | 1’37.191 | 3.478 / 0.081 |
| 19 | 77 | James ELLISON | GBR | Paul Bird Motorsport | ART | 283.5 | 1’37.281 | 3.568 / 0.090 |
| 20 | 22 | Ivan SILVA | SPA | Avintia Blusens | BQR | 284.3 | 1’37.554 | 3.841 / 0.273 |
| 21 | 5 | Colin EDWARDS | USA | NGM Mobile Forward Racing | Suter | 283.4 | 1’38.305 | 4.592 / 0.751 |
